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Title: Green Synthesis of Multifunctional Metal Oxide Nanoparticles from Medicinal Plants and their Biological Applications
Authors: Iqbal, Javed
Keywords: Plant Sciences
Issue Date: 2020
Publisher: Quaid-i-Azam University, Islamabad.
Abstract: Green synthesis of nanoparticles using medicinal plants has become a promising substitute to traditional physical and chemical approaches. In the current study, different metal oxide nanoparticles (MNPs) were successfully synthesized using one-pot, eco-friendly, green synthesis method using aqueous leaves extracts of medicinal plants Rhamnus virgata (RV), Geranium wallichianum (GW), Rhamnella gilgitica (RG). Further, the greenly orchestrated Fe2O3 (Iron oxide), NiO (Nickel oxide), ZnO (Zinc oxide) and Co3O4 (Cobalt oxide) nanoparticles were extensively studied using different microscopic and spectroscopic techniques such as UV-Vis (Ultraviolet-Visible), XRD (X‐ray diffraction), FT-IR (Fourier‐transform infrared), Raman, DLS (Dynamic light scattering), EDS (Energy-dispersive X-ray spectroscopy), SEM (Scanning electron microscopy) and TEM (Transmission electron microscopy). The average sizes of nanoparticles were found to be 20 nm (ZnO), 21 nm (Fe2O3), 24 nm (NiO), and 21 nm (Co3O4). Further, targated NPs were evaluated for different biological activities such as antibacterial, antifungal, anticancer, enzymes inhibition, antileishmanial, antioxidants and biocompatibility assays. Antibacterial potencies were investigated using different pathogenic bacterial strains via Disc diffusion method revealing significant antibacterial potentials. This method was used to confirm the antifungal activities using different fungal strains. Significant cytotoxicity potential was examined using brine shrimps cytotoxicity assay. Dose-dependent cytotoxicity assays were reported against Leishamnia tropica (Promastigotes and amastigotes) and HepG2 liver cancer cell line. In addition, the biosafe and nontoxic nature of targated nanoparticles was confirmed by biocompatibility assays using human RBCs and macrophages. Significant protein kinase inhibition was observed for RG@Fe2O3 nanoparticles while strong fungicidal and bactericidal activities were reported for RV@ZnONPs. Strong DPPH while good total antioxidants and total reducing powers were reported. Our experimental results concluded, that targated MNPs are biocompatible and nontoxic and can be used in different biomedical applications.
Gov't Doc #: 20529
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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