Please use this identifier to cite or link to this item: http://prr.hec.gov.pk/jspui/handle/123456789/14967
Title: Phytochemical investigation and biological screening of Euphorbia pulcherrima and Micromeria biflora
Authors: Humayun Khan, Muhammad
Keywords: Social Sciences
Education
Issue Date: 2020
Publisher: University of Peshawar, Peshawar.
Abstract: The research work in the present thesis deals with the phytochemical investigation, isolation, structure elucidation and pharmacological potential of Euphorbia pulcherrima and Micromeria biflora, which is presented in two parts. PART-A Part A deals with the study undertaken on the preliminary phytochemical analysis and bioassay-directed isolation of secondary metabolites of Euphorbia pulcherrima. The methanolic extract and its various fractions showed the presence of terpenoids, flavonoids, steroids and alkaloids while n-hexane, chloroform and ethyl acetate have fatty acids and steroids. Based on TLC profiles the chloroform fraction was selected for isolation of compounds. The chloroform fraction was subjected to column chromatography (CC) which resulted in the isolation of Spinacetin (1), Patuletin (2) and Hispidulin (3). The n hexane soluble fraction yielded three known hitherto reported compounds lupeol (4), β sitosterol (5), and stigmasterol (6) by employing column chromatography. Their structures were elucidated by advanced spectroscopic analysis. The extract, fractions and constituents were screened for various in-vitro and in-vivo activities. Compounds 1 and 2 showed promising urease, tyrosinase, antiglycation and phosphodiesterase activities. Based on the binding affinity and ADMET properties, the present study suggests that compounds 1 and 2 were more hydrophilic due to more hydrogen bonding, a high TPSA score. Moreover, evaluation of the ADMET parameters for the isolated compounds suggests good drug-like properties and opens the possibility for further optimization of studied compounds. The extract of the plant exhibited anti-nociceptive, sedative, anti inflammatory, antipyretic, acute toxicity, muscle relaxant and antipyretic activities against standard.part B deals with the study undertaken on the preliminary phytochemical composition and biological screening of M. biflora. The methanolic extract and fractions showed the presence of bioactive chemical constituents, such as terpenoids, flavonoids, caumarines and tannins which correlate the medicinal value of M. biflora. Based on the analysis of TLC the chloroform fraction was choose for isolation and was subjected to column chromatography (CC) which resulted in the isolation of compounds Salicylalazine (7). The n-hexane soluble fraction yielded two known hitherto β-sitosterol (8) and stigmasterol (9) by employing column chromatography. Their structures were elucidated by advanced spectroscopic analysis. The extract, fractions and constituents were screened for various in-vitro and in- vivo activities. Compound 7 showed promising urease, Tyrosinase, Antiglycation, and Phosphodiesterase activities. Based on the binding affinity and ADMET properties, the present study suggests that compounds 7 were more hydrophilic due to more hydrogen bonding, a high TPSA score. Moreover, evaluation of the ADMET parameters for the isolated compounds suggests good drug-like properties and opens the possibility for further optimization of studied compounds. The extract of the plant exhibited promising anti-nociceptive, sedative, anti-inflammatory, antipyretic, acute toxicity, muscle relaxant and antipyretic activities against standard.
Gov't Doc #: 20269
URI: http://prr.hec.gov.pk/jspui/handle/123456789/14967
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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