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Title: Identification Characterization and Bioevaluation of Polyphenols from Martynia annua Linn.
Authors: Muazzam, Saba
Keywords: Sciences
Issue Date: 2019
Publisher: Quaid-i-Azam University, Islamabad.
Abstract: Martynia annua L. is a medicinal plant found local to sub-continent where it is consumed for self-treatment of various diseases and ailments. The objectives of this study were to analyze different extracts of plant parts using untargeted and targeted metabolic profiling, identification/quantitation of selected compounds and evaluation of antioxidant and cytotoxic activities of all plant part’s extracts. A validated LC-MS/MS method was used for untargeted and targeted analysis of a range of extracts from the plant. Individual plant metabolites were identified/quantified by comparison with authentic standards, published fragmentation profiles and online database matching. For antioxidant activity, three different assays were used. Tetrazolium reduction method was employed to evaluate cytotoxic activity. A total of 89 compounds were identified and their relative abundances were estimated across five separate parts of the plant (leaves, stem, fruits, flowers and roots). Sixteen of these compounds were identified by comparison with authentic standards and were subsequently ascertained in all plant parts. The identity of 18 glycosides compounds were determined by deduction engaging accurate mass, isotope pattern and experimental collision induced dissociation fragmentation spectra. Fifty five compounds were identified putatively by matching with HMDB (Human Metabolome Database). A number of compounds identified at higher abundance which include: homovanilic acid, syringic acid, trans-ferulic acid, apigenin, luteolin, hispidulin and isorhamnetin have biological properties and also estimated their concentration and independently determined biotherapeutic properties. The plant extracts showed significant antioxidant and cytotoxic activity. These findings significantly extend the plant metabolites identified in the Martynia annua. These results anticipate a biochemical support for future studies addressing bio-therapeutic potential of this plant.
Gov't Doc #: 19748
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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