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Title: Chelant assisted lead (Pb) phytoextraction and its subsequent role in antioxidant system of leafy and rooty vegetables
Authors: Khan, Imran
Keywords: Botany
Issue Date: 2016
Publisher: Government College University, Faisalabad
Abstract: The experiments were conducted to explore the chelant-assisted lead (Pb) phyto-extraction and subsequent antioxidative responses in spinach and turnip plants under both controlled (Growth Incubator) environment and filed (natural) conditions. The seeds of turnip (Brassica Rapa L. cv. Purple Top) and spinach (Spinacea oleracea L. cv. Desi) were surface sterilized and sown in plastic pots filled with thoroughly washed sand. Later on, the same experiment was conducted in the field under natural conditions. In controlled experiment, after germination, 10 d old spinach and turnip seedlings were exposed to different Pb regimes (0, 2.42 and 4.83 mM). While in case of field experiment, 20 d old plants were expose to Pb regimes where the control plot exhibited 0.0218 mM Pb concentration. After 10 days of Pb application, 2.4 mM concentrations of different chelating agents viz., ethylenediamine tetra acetic acid (EDTA), citric acid (CA), oxalic acid (OA), tartaric acid (TA) and malic acid (MA) were applied to the plants both under controlled and field conditions. Different Pb regimes altered biomass and different physiobiochemical attributes in both crop species. On the other hand, the application of chelates, especially CA increased growth in both crop species. However, of the chelates, TA was much more effective in increasing tissue specific Pb concentrations in both crops. The beneficial effects of chelates attributed to the capacity to increase photosynthetic pigments, antioxidative enzymes and nutrient uptake under different Pb regimes. Both crop species were able to tolerate Pb levels applied. Furthermore, some chelates were able to reduce tissue specific toxic concentration of Pb leading to hormesis. Overall, the results elaborated the potential of chelates in increasing growth, biomass, and thus suggested their use for phytoextraction of Pb. Based on the effectiveness of TA in increasing root Pb concentrations in case of turnip and shoot Pb concentrations in case of spinach suggested its use for phytoextraction of Pb in Pb-polluted environments.
Gov't Doc #: 15185
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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