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Title: Phytochemical Investigation of Seriphidium and Caragana Brachyantha
Authors: Ali, Liaquat
Keywords: Physical Sciences
Organic Chemistry
Issue Date: 2021
Publisher: Islamia University, Bahawalpur.
Abstract: Throughout the journey of mankind, the reliance of man on plants to alleviate sufferings is still considered with more awareness for their therapeutic applications. Dependence on traditional medicinal system is because of its less cost, safer and easy accessibility. Therefore, in the modern world, interest in the discovery of drug candidates derived from the chemical constituent of plant is an important element of naturopathy. In connection of this interest, the under discussion study describes phytochemical investigation of two medicinal plants: Seriphidium oliverianum and Caragana brachyantha. The work done during this study is embodied in this dissertation as six chapters. The introductory portion and background information on the topic has been provided in chapter 1. Since phytochemical investigations resulted in the isolation of flavonoids, steroids and alkaloids from these two plants, the biosynthesis of these classes has also been discussed and incorporated in chapter 2, whereas, chapter 3 describes the previous phytochemical investigation of the genera Seriphidium and Caragana, besides this, it offers a literature survey on Seriphidium oliverianum and Caragana brachyantha, the two target species. Chapter 4 elaborates the biological screening of various extracts of Seriphidium oliverianum and Caragana brachyantha; they were screened in several bioassays including DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, metal chelation, α-glucosidase, α amylase , tyrosinase and were also analyzed for their total phenolic and flavonoid contents. Among different polarity solvent extracts (methanol, hexane, ethyl acetate and water) methanol extract of Seriphidium oliverianum (So-M) offered the most convincing results in the DPPH, ABTS, CUPRAC and FRAP antioxidant assays which are in accord to the highest phenolic and flavonoid contents in So-M. While in enzyme inhibition assays Seriphidium oliverianum hexane extract (So-H) was found more active in tyrosinase and α amylase activities. This part also portrays the isolated compounds obtained as a result of chromatographic purification of the extract of n-hexane and the ethyl acetate soluble fractions of methanolic extract of Seriphidium oliverianum. Hexane extract provided three new phytochemicals; named as diethyl-2,6-diphenyl-(3-nitrophenyl)-1, 4-dihydropyridine-3, 5-dicarboxylate (267), 6-diacetyl-3, 5-dimethyl-(3-nitrophenyl)-1, 4-dihydropyridine (268) and diethyl-2,6-dimethyl-4-(3-nitrophenyl)-1,4dihydropyridine-3, 5-dicarboxylate (269) xiv along with four known (270-73) compounds while ethyl acetate extract yielded eleven (130, 133, 134, 136, 154, 160, 274-278) known secondary metabolites. On the other hand, hexane, ethyl acetate, butanol, methanol and water extracts of Caragana brachyantha were also screened where the ethyl acetate extract (Cb-E) showed the highest antioxidant potential in DPPH, ABTS, CUPRAC, FRAP and phosphomolybdenum assay, results were proportional to its higher total phenolic and flavonoid contents. The structure elucidation of isolated compounds (162, 167- 171, 306- 307) with their spectroscopic data is also explained in this part of results and discussion. Experimental procedures employed in this study, plant extraction, isolation procedure, along with spectroscopic data are discussed in chapter 5. Bioassay protocols are included in chapter 6.
Gov't Doc #: 23680
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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