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Title: Effect of self-regulated learning strategies on students' achievement and motivation for learning English in public sector elementary schools in Lahore.
Authors: , Umm e Habiba
Keywords: Social Sciences
Issue Date: 2018
Publisher: University of the Punjab , Lahore
Abstract: The increasing prevalence of hepatic disorders has become a major challenge for the scientists. Medicinal plants have remarkable potential to cure liver diseases by reducing reactive oxygen species that induce oxidative stress to the cells. Therefore, the present study was planned to prepare a herbal formulation that may prevent and manage several liver disorders and its related complications. This study was accomplished in three phases. In phase I, in vitro screening of indigenous medicinal plants was done on the basis of their antioxidant, toxicological and hepatoprotective potential. The total phenolic and flavonoid content were found in the range of 67.02 to 267.43 GAE and 73.26 to 131.86 CE respectively. The antioxidant potential of medicinal plants was explored through ferric reducing antioxidant power assay, DPPH and hydroxyl radical scavenging activities. The obtained results showed 33.04 to 84.73% DPPH inhibition, 32 to 94% hydroxyl radical scavenging activity and 10.14 to 44.49 ferric reducing antioxidant power at 100, 300 and 500 µg/mL concentrations. The antioxidant potential of studied medicinal plants increased in dose dependent manner. For toxicity evaluation of medicinal plants, hemolytic and thrombolytic activities were performed in terms of percentage hemolysis and clot lysis respectively. Medicinal plants that showed minimum percentage hemolysis and clot lysis were considered best and safe. The hepatoprotective potential of medicinal plants was exhibited through acetylcholinesterase (AChE) inhibition assay and Liver Slice culture (LSC) assay. Medicinal plants that showed maximum hepatoprotective potential in terms of minimum inhibition of AChE activity and minimum percentage cytotoxicity were selected for phase II. In phase II, biochemical profiling of selected plants was done through LC-MS to detect and confirm the presence of hepatoprotective bioactive compounds. Moreover, different herbal combinations of selected plants suggested by Response surface methodology (RSM) were prepared and analyzed through in vitro Liver Slice Culture assay in phase II. The herbal combination that showed minimum percentage cytotoxicity in terms of maximum hepatoprotective potential was selected for phase III, in vivo studies. In this phase, rats were used as animal model to evaluate the ameliorative effects of selected herbal combination against hepatotoxicity. The heaptotoxicant CCl4 with the dose of 1 mL/kg b.wt was used to induce hepatotoxicity and herbal combination with the dose of 200 mg/kg b.wt was used as curative and preventive mode of treatments for thirty days. Biochemical parameters including ALT, AST, ALP, ƔGT, total bilirubin, total protein, albumin and globulin were measured in blood serum of all groups. Percentage of acetylcholinesterase enzyme activity, malondialdehyde (MDA) in terms of lipid peroxidation and antioxidant enzymes SOD and GSH were measured in liver homogenate of rats. Liver histopathological analysis was done to see any alteration in hepatic cells due to exposure of heaptotoxicant CCl4 and then healed with herbal combination in curative and preventive mode of treatments. The significant decrease in ALT, AST, ALP, ƔGT, total bilirubin, MDA and increase of total protein, albumin, globulin, AChE activity SOD and GSH levels in both curative and preventive treatment groups as compared to positive control groups indicated significant ameliorative effects of herbal formulation. Likewise histopathological examination with normal architecture of hepatic cells in curative and preventive treatment groups endorsed the hepatoprotective potential of herbal combination. Therefore, this herbal combination might be used as an alternative medicines against various liver disorders and its related complications
Gov't Doc #: 23350
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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