Please use this identifier to cite or link to this item: http://prr.hec.gov.pk/jspui/handle/123456789/17006
Title: Molecular identification, characterization and transmission studies in oilseed crops associated phytoplasma and their insect vectors
Authors: Aslam, Muhammad
Keywords: Agriculture and Veterinary Sciences
Entomology
Issue Date: 2021
Publisher: University of Agriculture, Faisalabad.
Abstract: Phytoplasma inhabiting sieve elements of plants are potentially destructive plant pathogens which are the main causual agents of phytoplasma diseases transmission. These plant pathogens are transmitted from one plant to another plant via insect vectors like leaf hoppers, tree hoppers and plant hoppers. They are also transmitted through grafting, propagation material (seeds) and dodder plant species. The diseases caused by phytoplasma are destructive in nature and has been reported to cause substantial yield losses by restricting the growth of plants and modifying their floral strctures into leafy structures which result in no seed formation. The current study was conducted to examine etiology, taxonomy, symptomatology and transmission of phytoplasma infecting oilseed crops (brassica species, sunflower and sesame) in Pakistan and their associated insect vectors. Experiments were carried out in "Dr. Jam Laboratory" Department of Entomoogy, University of Agriculture Faisalabad. Infected samples of Brassica, Sesame were collected from Rahim Yar Khan, Khanewal, Dera Ghazi Khan and Faisalabad districts of Punjab during a survey 2015-2019, Pakistan. Infected plants showed leaf reddening or yellowing, hypertrophied calyxes, floral abnormalities, greening of petals, sepal hypertrophy and abnormal shoot proliferation. Diseased plants expressing characteristic symptoms was confirmed by Polymerase chain reaction (PCR) using 16Sr DNA and tuf DNAs, followed by Restriction Fragment Length Polymorphism (RFLP) and sequence comparison of tuf genes and 16S rRNA. The results indicated that 16SrIX-H phytoplasma strain was identified for the first time as new subgroup associated with sarson (Brassica compestris) disease in Pakistan. Presence of phytoplasma was also detected via microscopy and transmission trias using dodder and insect vector Orosius albicinctus. For sunflower and sesame same molecular and transmission tests were used to identify phytoplasma group and subgroup. Detected phytoplasma in sunflower samples belonged to ‘16SrII-D while on sesame 16SrIX-H and 16SrII-D phytoplasmas were identified in infected plant samples. The insect vectors, Orosius albicinctus, Orosius argentatus in sesame crop while Circulifer tenellus, Orosius argentatus in sunflower crop were shown as confirmed vectors for phytoplasma transmission. The further field survey are needed to identify these 16SrIX-H and 16SrII-D phytoplasmas in other agricultural crops. These phytoplasma associated diseases are managed by developing resistant varieties and proper control of insect vectors
Gov't Doc #: 23251
URI: http://prr.hec.gov.pk/jspui/handle/123456789/17006
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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