Please use this identifier to cite or link to this item: http://prr.hec.gov.pk/jspui/handle/123456789/16680
Title: Evaluation of Antioxidant Status and their Enhancements Strategies for Drought Tolerance in Chickpea Under Agro-Ecological Conditions of Southern Punjab
Authors: Yousaf, Muhammad
Keywords: Agriculture and Veterinary Sciences
Agronomy
Issue Date: 2021
Publisher: Bahauddin Zakariya University Multan
Abstract: Water stress is one of the major factors restricting the growth and development of chickpea plants by inducing various morphological and physiological changes. Therefore, a set of four field experiments was designed to evaluate the antioxidant status and their enhancements strategies for water stress tolerance in chickpea on loam and clay loam soils under agro-ecological conditions of Arid Zone Research Institute, Bahawalpur and Cholistan farm near Derawer Fort Cholistan desert of Bahawalpur, Southern Punjab, Pakistan during Rabi 2013-14 and 2014-15. In the first study, experimental treatments comprised of five approved chickpea cultivars (Thall 2006, DUSHT, Punjab 2008, Bakhar 2011, and AZC 06) were selected to screen out for their drought tolerance under seven water stress levels (water deficit at flowering stage, water deficit at pod formation stage, water deficit at grain filling stage, water deficit at flowering + pod formation stage, water deficit at flowering + grain filling stage, water deficit at pod formation + grain filling stage and water deficit at flowering + pod formation + grain filling stage) including well-watered (control). Results indicated that water stress at various growth stages adversely affects the growth, yield, and quality attributes of all chickpea cultivars. However, the chickpea cultivar Bakhar 2011 showed promising antioxidant enzymatic activity under water stress at various growth stages and produced significantly higher growth and yield contributing attributes. Whereas chickpea cultivar DUSHT exhibited poor performance under water stress conditions. In the second study, experimental treatments comprised of two chickpea cultivars i.e. Bakhar 2011 (drought tolerant) and DUSHT (drought sensitive), two water stress levels i.e. water stress at flowering stage and water stress at flowering + pod formation + grain filling stage including well-watered (control) and two exogenous application of osmoprotectants i.e. glycine betaine (GB) 20 ppm and proline 10 uM including distilled water (control). Results indicated that the exogenous application of GB improved the growth, yield, and quality parameters of both chickpea cultivars even under water stress conditions. Our results suggested that the application of GB mitigates the adverse effects of water stress and enhanced tolerance in chickpea mainly due to higher antioxidant enzyme activity, demonstrating the protective measures of plant cells in stress conditions. In third study, experimental treatments comprised of two chickpea genotypes i.e. Bakhar 2011 (drought tolerant) and DUSHT (drought sensitive), two water stress levels i.e. 2 water stress at flowering stage and water stress at flowering + pod formation + grain filling stage including well watered (control) and three exogenous application of nutrients i.e. KCl 200 ppm, MgCl2, 50 ppm and CaCl2, 10 mM including distilled water (control). Results indicated exogenous application of potassium chloride improved the growth, yield, and antioxidant enzyme activities of both chickpea genotypes even under water stress conditions. In the fourth study, experimental treatments comprised of two chickpea genotypes i.e. Bakhar 2011 (drought tolerant) and DUSHT (drought sensitive), two water stress levels i.e. water stress at flowering stage and water stress at flowering + pod formation + grain filling stage including well watered (control) and application of organic manures i.e., farmyard manure (FYM): 20 tons ha-1 and press mud: 15 tons ha-1 either alone or in combination in addition to control. Results indicated that water deficit at distinctive growth stages adversely affected the growth, yield and quality attributes of both chickpea cultivars. Combined application of FYM and press mud produced superior growth and yield contributing attributes while maximum antioxidant enzyme activities were observed in drought tolerant genotype (Bakhar 2011) under well-watered conditions and under water stress at flowering + pod formation + grain filling stage, respectively. These results suggested that the combined application of organic manure mitigated the adverse effects of water stress as its decomposition incorporated micro and macronutrients into the soil.
Gov't Doc #: 23087
URI: http://prr.hec.gov.pk/jspui/handle/123456789/16680
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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