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Title: Clearance of bilirubin by use of phototherapy, gamma irradiation and leaf extract of Cichorium intybus L. in experimental rabbits
Authors: Nawaz, Haq
Keywords: Biological & Medical Sciences
Issue Date: 2020
Publisher: Bahauddin Zakariya University Multan
Abstract: Hyperbilirubinemia is a clinical condition characterized by elevated levels of bilirubin in the blood due to failure in its transport, conjugation, and excretion. Among the current methods of treatment, phototherapy is the accepted and widely used method for the management of hyperbilirubinemia. This process has some limitations due to prolonged exposure time, slow rate of recovery, and chances of rebinding of bilirubin to albumin. The present study was, therefore, planned to investigate a new method of bilirubin clearance by the use of low-dose gamma irradiation. In the first phase of the study, induction of hyperbilirubinemia in experimental rabbits by phenyl hydrazine was optimized in terms of dose, number of doses and dose interval using response surface methodology. A 3-factorial Central Composite Design was employed using five levels for each of the three input variables. The degree of hyperbilirubinemia was measured in terms of elevation in bilirubin level in serum of the experimental rabbits. A dose dependent significant elevation (p<0.05) in serum total bilirubin level was observed after PH treatment. Optimum levels of phenyl hydrazine dose, total number of doses and a dose interval to achieve maximum elevation in serum total bilirubin (4.06 mg dL −1 ) were found to be 11.56 mg kg−1 body weight, 8 and 24.65 h, respectively. In the second phase of the study, hyperbilirubinemia was induced in rabbits using phenyl hydrazine at optimum levels of induction variables (Total serum bilirubin: 2.94-4.057 mg dL-1 ) followed by the treatment with UV light, Cichorium intybus leaf extract, and gamma radiation. Each of the treatments methods showed a significant (p<0.05) dose-dependent increase in total serum bilirubin clearance from the animal body. The dose dependent clearance of hyperbilirubinemia was found to be comparatively highest (32-75%) in gamma irradiation followed by UV treatment (27- 56%) and lowest in herbal treatment (28-32%). The sera obtained from the control and gamma irradiated rabbits were also analyzed for liver function enzyme including Glutamate pyruvate transaminase, Glutamate oxaloacetate transaminase and alkaline phosphatase and hematological parameters including hemoglobin level, and red blood cells, total leukocyte and platelet count. The observed values of hematological parameters and enzymes level of the control group before and after gamma irradiation were found to be in normal ranges. However, the phenyl hydrazine treatment resulted in a decrease in vi hematological parameters and an increase in the level of liver function enzymes in hyperbilirubinemic rabbits which were found to be recovered to normal after gamma irradiation. In the third phase of the study, HPLC analysis of bilirubin solution showed a dose dependent exponential decrease in concentration of bilirubin and an increase in its in vitro gamma degradation products. The HPLC analysis of chloroform extracts of sera obtained from the gamma irradiated rabbits also showed a dose dependent exponential decrease in concentration of bilirubin and an increase in its in vivo gamma radiolysis products. The developed HPLC method was found to be accurate and precise with good signs of reproducibility and repeatability. In conclusion, the study provides the optimum conditions for induction of hyperbilirubinemia and a fast, secure and more reliable method of treatment of hyperbilirubinemia by the use of low-dose gamma radiation. The study also provides a validated HPLC method for the determination of bilirubin and its degradation products. However, the identification of the observed degradation products of bilirubin still needs to be investigated
Gov't Doc #: 22974
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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