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Title: Toxicity Evaluation of Titanium Dioxide Nanoparticles and Remedial Effect of Cinnamomum cassia ana Azadirachta indica in the Brain and Testis of Sprague Dawley Rats
Authors: Latif, Muhammad Asif
Keywords: Biological & Medical Sciences
Issue Date: 2021
Publisher: Government College University, Faisalabad
Abstract: Titanium dioxide nanoparticles (TiO2-NPs) are extensively used metal oxide nanoparticles in nanomedicines, semiconductor, paints, industrial photo-catalytic processes, cosmetics, food industry, rubber industry, sunscreen lotions and pharmaceutical industry. Due to the extensive use of TiO2 nanoparticles (NPs) in the industry field and on going commercialization of nanotechnology products, the exposure of the human body intentionally or unintentionally to nanoparticles is possible and may continue to increase. Therefore, the assessment of its potential toxicity and distribution in the body, as well as the remedial activity of natural and easily available antioxidants is essential. Many medicinal plants with antioxidant properties are reported to mitigate the toxic effect of nanoparticles induced toxicity. The current study investigated the toxic effect of TiO2-NPs and remedial effect of Cinnamomum cassia and Azadirachta indica in brain and testis of male Sprague Dawley rats. Experiment consist of 2 phases. In the 1st phase, twenty five adult male spargue Dawley rats grouped into five groups (n=5) named as nontreated, placebo group (treated with normal saline) and three treated groups named as G1, G2 and G3 intraperitonealy treated with TiO2-NPs @ 80, 120 and 160mg/kg body weight for 28 days. In 2nd phase, 60 post weaning male Sprague Dawley rats weighing 200+10g were grouped 12 groups having 5 (n=5). Toxicity was induced in the rats using sub-lethal doses of TiO2-NPs (120mg/kg BW) and mitigated by using three different doses (100, 150 and 300mg/kg BW) of C.cassia or A. indica or in combined form for 90 days. TiO2-NPs were characterized by (XRD) and (VP-SEM). C. cassia and A. indica extracts were subjected to phytochemicals analysis, proximate analysis and mineral analysis to determine the quantitative and qualitative analysis of secondry metabolities, macro nutrients and mineral composition. At the end of experiment, rats were anaesthetized, sacrified and selected organ (Brain and Testes) were isolated. Accumulation of titanium in selected organ (Brain & Testes) was analyzed by ICP-MS. Percentage of cell viability in selected organs was analyzed by micro plate reader. Genotoxic effect of TiO2-NPs induced toxicity was determined through comet assay. Histological studies were observed on the tissue of selected organ (Brain and Testes) exposed to toxicity. Serum testosterone level was determined using ELIZA test kit. Histometric method of sperm count was done by sperm count. Results showed TiO2-NPs xviii have 25-55nm range in size with spherical morphology. Body weight and sometic index of brain and testis was significantly (P<0.05) decreased in higher treated groups (160mg/kg BW) as compared to non-treated group. Hematological parameter such as WBC, MCH, RDWc, PLT, MPV, PDWc were significantly (p<0.05) increased while RBC, LMY, MID, GRA, HGB, HCT, MCV, MCHC and PCT were significantly (p<0.05) decreased in high dose treated group (G3) as compared to other treated and non treated group.Testosterone level, Sperm count percentage of cell viabiltyand activity of acetylcholine esterase significantly (p< 0.05) decreased in a dose dependent manner. Similarly, length of head and head DNA (%) was significantly (p<0.05) decreased while length of tail, length of commet and tail DNA (%) were significantly (p<0.05) increased in brain and testis in a dose dependent manner. Moreover, investigated alterations were helpful in determining the toxic effect of TiO2-NPs in targated organ. Administration of C.cassia and A. indica significantly ameliorated TiO2-NPs induced changes. However, more ameliorative effect were observed with A. indica extract at high dose due to high amount of its secondary metaboloties
Gov't Doc #: 22842
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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