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Title: Formulation, characterization and pharmacokinetics of nanoparticle based preparations of gentamicin
Authors: Akhtar, Bushra
Keywords: Biological & Medical Sciences
Issue Date: 2019
Publisher: University of Agriculture, Faisalabad.
Abstract: Parenteral preparations are considered costly therapy, painful drug delivery as well as the risk of infections at administration site. This unlocks the panorama for development of new methods regarding delivery of such drugs. Gentamicin (GM) is a renowned aminoglycoside and is the drug of choice for several diseases. Currently, it is available in parenteral formulations and few topical preparations are also available that are not meant for systemic drug delivery. Keeping in mind the above scenario and to fulfill the need of alternative dosage routes, the current research was conducted. Two controlled release preparations of gentamicin (GM) were prepared by using poly lactic co glycolic acid (PLGA) nanoparticles (NPs) i.e. oral and transdermal patch to achieve systemic drug concentrations. The oral formulation of gentamicin loaded PLGA nanoparticles were surface modified by coating it with chitosan. Similarly, GM loaded PLGA NPs were also prepared which were later used in transdermal patch. The NPs were characterized by zeta potential and polydispersity index (PDI), entrapment efficiency, scanning electron microscopy (SEM), X-rays diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis. Transdermal patches were characterized further for various physical parameters. Analysis of variance (ANOVA) was applied for hematological and biochemical parameters by using Graph pad prism software. In vitro studies following in vivo pharmacokinetics were conducted. Pharmacokinetic parameters were determined by using APO software. Toxicity studies of these formulations, including hematological, biochemical parameters and histopathological studies of were carried out. No alterations were observed in any of the above-mentioned tests. Various pharmacokinetic parameters determined for oral and transdermal patch determined were the area under the curve (AUC), time to reach maximum concentration (Tmax), maximum concentration (Cmax), volume of distribution (Vd) and clearance. Based on the above results, it is concluded that both the formulations have potential for systemic absorption of Gentamicin and can be used for achieving therapeutic benefits. However, further studies are required for dose optimization of the formulations.
Gov't Doc #: 20285
Appears in Collections:PhD Thesis of All Public / Private Sector Universities / DAIs.

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